Type
Blood Testing
Biomarkers
16
Duration
30 min
Results
24 hours
Sample
Blood (venous draw)
What's included
6 deliverables in this treatment
- Vitamin D (25-OH) — immunochemistry on DPC Immulite 2000XPi
- Vitamin B12 (cobalamin) — nerve and red cell health marker
- Folate (folic acid) — one-carbon metabolism and red cell marker
- Ferritin — most sensitive iron-storage marker
- Magnesium — muscle, nerve, and metabolic cofactor
- Printed and electronic results same or next working day
A five-marker nutritional deficiency screen covering the vitamins and minerals most commonly deficient in Central European adults — Vitamin D, Vitamin B12, folate, ferritin, and magnesium. At PKBH Olomouc, Vitamin D and B12 are measured by immunochemistry on the DPC Immulite 2000XPi or Abbott Alinity ii, folate by chemiluminescence, and ferritin on the Mindray BS-800 M2 biochemistry line. Vitamin D was added to the PKBH test menu in May 2023 following patient demand. Walk-in access, no referral required.
About this treatment
Nutritional deficiencies are among the most under-recognised causes of fatigue, cognitive difficulty, mood disturbance, musculoskeletal pain, and immune vulnerability in adults. Unlike dietary questionnaires, blood-based quantification provides objective values that guide precise supplementation and track response over time.
Five markers and their clinical significance: - Vitamin D (25-hydroxyvitamin D) — the primary circulating form of vitamin D, measured to determine deficiency (< 50 nmol/L), insufficiency (50–75 nmol/L), or adequacy (> 75 nmol/L). Vitamin D regulates calcium absorption, immune T-cell differentiation, muscle function, and has been associated with reduced risk of respiratory infections, autoimmune conditions, and depression. At Central European latitudes,…
All five tests draw from one venous EDTA or serum tube collected at a PKBH Olomouc site. Results are issued electronically and in print on the same day or the next morning.
Good to know
- Biomarkers
- 5 (Vitamin D, B12, folate, ferritin, magnesium)
- Vitamin D platform
- DPC Immulite 2000XPi
- Results
- Same day / next morning
- Referral needed
- No — walk-in self-pay
- Vitamin D added
- May 2023
Who is this for?
Chronic fatigue, vegans/vegetarians, osteopenia, heavy periods, endurance athletes, adults over 60, winter Vitamin D screening
Preparation required
No fasting required. Avoid high-dose biotin supplements for 12 hours before testing. Walk in from 07:00 — no appointment needed.
Panel categories
Biomarkers tested
16An essential trace mineral in its trivalent form (Cr³⁺), important for insulin signalling and glucose metabolism. Hexavalent chromium (Cr⁶⁺) from industrial exposure is carcinogenic. Testing measures total chromium levels.
A trace mineral essential for iron metabolism, connective tissue formation, and antioxidant defense. The copper/zinc ratio is an emerging marker of inflammation and oxidative stress. Both deficiency and excess can cause problems.
Essential for DNA synthesis, cell division, and red blood cell formation. Folate deficiency causes megaloblastic anemia and, during pregnancy, increases the risk of neural tube defects. Works closely with vitamin B12.
Essential for thyroid hormone production and overall metabolic regulation.
Involved in over 300 enzymatic reactions including energy production, muscle function, and nerve signaling. Magnesium deficiency is common and associated with muscle cramps, anxiety, insomnia, and increased cardiovascular risk.
An essential trace mineral needed for bone formation, blood clotting, and antioxidant defense (via superoxide dismutase). Excess manganese, typically from occupational inhalation, causes manganism — a Parkinson's-like neurological condition.
Trace mineral involved in enzyme function and metabolism.
A trace mineral crucial for thyroid hormone metabolism, antioxidant defense (via selenoproteins), and immune function. Selenium deficiency can impair thyroid function and increase susceptibility to viral infections.
A fat-soluble vitamin essential for vision, immune function, skin health, and cell growth. Both deficiency and excess can cause health problems. Levels are tightly regulated by the liver.
Essential for nerve function, DNA synthesis, and red blood cell formation. B12 deficiency can cause anemia, neuropathy, fatigue, and cognitive impairment. Common in vegans, vegetarians, older adults, and those taking metformin or acid-reducing medications.
A powerful water-soluble antioxidant essential for collagen synthesis, immune function, and iron absorption. Vitamin C deficiency causes scurvy. It also supports wound healing and protects against oxidative stress.
The best indicator of overall vitamin D status. Vitamin D is essential for calcium absorption, bone health, immune function, and mood regulation. Deficiency is extremely common, especially in northern latitudes, and linked to increased disease risk.
A fat-soluble antioxidant that protects cell membranes from oxidative damage. Vitamin E works synergistically with vitamin C and selenium. Deficiency is rare but can cause nerve and muscle damage.
Essential for blood clotting and bone metabolism. Vitamin K activates proteins needed for blood coagulation and calcium regulation in bones. Deficiency increases bleeding risk and may contribute to osteoporosis.
An essential trace mineral involved in immune function, wound healing, DNA synthesis, and testosterone production. Zinc deficiency impairs immune response, delays wound healing, and can cause hair loss and taste changes.
About the clinic
Reviews, team and credentials
Independent review links and clinic-reported team information.
Clinical team
PKBH is led by MUDr. Milada Popotrandovská as director, with analytical operations headed by RNDr. Ivana Slouková and quality management overseen by Mgr. Vojtěch Mutina. The haematology department is supervised by MUDr. Jaromír Hubáček and doc. MUDr. Jaroslav Bačovský, CSc. Metrological oversight is held by RNDr. Kateřina Mutinová, and the Prostějov site is headed by doc. MUDr. Josef Bartek, CSc. All laboratory…
Languages: cs
Treatment highlights
- Vitamin D (25-OH) — immunochemistry on DPC Immulite 2000XPi
- Vitamin B12 (cobalamin) — nerve and red cell health marker
+4 more items in What's included
Approx. 1,402 Kč per panel (Vitamin D 502 + Vitamin B12 278 + Folate 275 + Ferritin 249 + Magnesium 23 Kč = 1,327 Kč, plus 75 Kč venous draw and serum separation). Tests billed à la carte at published PKBH self-pay rates. Vitamin D was added to PKBH's menu in May 2023. No online booking — walk-in only; no appointment required. Results same or next working day.