Type
Blood Testing
Duration
20 min
Results
24 hours
Sample
Blood (venous draw)
What's included
5 deliverables in this treatment
- Anti-Borrelia IgM — acute/recent infection marker
- Anti-Borrelia IgG — past or persistent infection marker
- ELISA method (first tier of two-tier CDC/EUCALB protocol)
- Printed and electronic results same or next working day
- Confirmatory IgG and IgM Western blot available at same laboratory (additional cost)
A two-tier Lyme disease serology screen covering Anti-Borrelia burgdorferi IgG and IgM antibodies by ELISA — the first line of the CDC/EUCALB two-tier testing algorithm for Lyme borreliosis. PKBH Olomouc offers this self-pay without a referral, with results same or next working day. If the ELISA is positive or equivocal, confirmatory IgG and IgM Western blot tests are available at the same laboratory, completing the two-tier protocol without changing provider.
About this treatment
Lyme borreliosis is the most common tick-borne infectious disease in Central Europe, and the Czech Republic's forested terrain — including the Jeseníky range surrounding Olomouc — creates high tick exposure risk throughout spring, summer, and autumn. Accurate serological diagnosis requires a two-step testing approach endorsed by the CDC, EUCALB, and the Czech infectious disease guidelines.
The two-tier protocol
- Step 1 — ELISA (first line): Anti-Borrelia IgM and IgG antibodies are measured by enzyme-linked immunosorbent assay. IgM appears from approximately 2–4 weeks after tick bite and indicates recent or acute infection; IgG develops from 4–6 weeks and persists for months to years. A negative ELISA in a patient with symptoms lasting fewer than 30 days after a tick bite does not exclude early Lyme disease — serology may not yet be positive. An equivocal or positive result on Step 1 triggers Step 2.
- Step 2 — Western blot (confirmatory): Anti-Borrelia IgG blot and/or IgM blot run on the same or a subsequent sample. The blot identifies the specific Borrelia proteins recognised by the patient's antibodies, filtering out false positives that can occur with ELISA due to cross-reactivity with other infections (EBV, CMV, ANA). PKBH offers both IgG and IgM blots at 2,835 Kč each — available as an add-on or on a return visit.
Good to know
- Biomarkers
- Anti-Borrelia IgG + IgM (ELISA)
- Protocol
- Two-tier CDC/EUCALB (blot available for step 2)
- Results
- Same day / next morning
- Referral needed
- No — walk-in self-pay
- Confirmatory blot
- Available at PKBH (2,835 Kč/blot)
Who is this for?
Recent tick bite with symptoms, post-tick fatigue or joint pain, unexplained neuropathy in endemic area residents, post-Lyme treatment monitoring
Preparation required
No fasting required. Walk in from 07:00 at any PKBH Olomouc site — no referral needed for self-pay. Confirmatory Western blot can be added at the same visit if the ELISA result is equivocal.
About the clinic
Reviews, team and credentials
Independent review links and clinic-reported team information.
Clinical team
PKBH is led by MUDr. Milada Popotrandovská as director, with analytical operations headed by RNDr. Ivana Slouková and quality management overseen by Mgr. Vojtěch Mutina. The haematology department is supervised by MUDr. Jaromír Hubáček and doc. MUDr. Jaroslav Bačovský, CSc. Metrological oversight is held by RNDr. Kateřina Mutinová, and the Prostějov site is headed by doc. MUDr. Josef Bartek, CSc. All laboratory…
Languages: cs
Treatment highlights
- Anti-Borrelia IgM — acute/recent infection marker
- Anti-Borrelia IgG — past or persistent infection marker
+3 more items in What's included
Approx. 851 Kč for first-line ELISA screen (Anti-Borrelia IgG 388 + Anti-Borrelia IgM 388 Kč = 776 Kč, plus 75 Kč venous draw and serum separation). If first-line is positive or equivocal, confirmatory Western blot (IgG blot + IgM blot) is available at 2,835 Kč each — a second visit or add-on. Tests billed à la carte at published PKBH self-pay rates. No online booking — walk-in only; no appointment required.