ALEX Allergy Xplorer — 280+ Allergen Multiplex Panel

The ALEX Allergy Xplorer panel is one of the most comprehensive single-draw allergy blood tests available in Europe, testing sensitisation to more than 280 allergen components from a single venous sample. Unlike skin-prick tests or standard single-allergen IgE panels, ALEX2 uses multiplex luminescence immunoassay technology to measure specific IgE antibodies at the molecular component level — identifying exactly which protein within a pollen, mite, or food is triggering a response. At Laboratoře Mikrochem, this analysis is performed in their ISO 15189-accredited immunology laboratory in Olomouc, with results available within 7 working days. The panel covers all major inhalant allergens (grass, tree, and weed pollens; dust mites; moulds; animal epithelia), insect venoms, and a broad food allergen set including milk proteins (casein, whey), egg, wheat gluten, soy, tree nuts, peanuts, shellfish, and tropical fruits. A single venous blood draw is all that is needed — no repeated skin punctures, no allergen exposure on the day.

The ALEX Allergy Xplorer (ALEX2) panel is a state-of-the-art multiplex allergy diagnostic that tests specific IgE antibody levels against more than 280 individual allergen molecules from a single tube of blood. It is among the most comprehensive allergy blood tests commercially available and is increasingly used by allergologists across Central Europe to replace or supplement conventional skin-prick testing — particularly for patients with complex or multi-sensitised allergy profiles. At Laboratoře Mikrochem's ISO 15189-accredited Olomouc laboratory, the ALEX2 assay uses luminescence-based multiplex immunoassay technology: allergen components are arrayed on a solid-phase matrix and incubated with the patient's serum; a fluorescent anti-IgE antibody then reveals which specific molecules triggered IgE production. The result is a quantified IgE titre for each component, expressed in kUA/L, allowing the allergologist to distinguish between primary sensitisation (the actual trigger) and cross-reactive sensitisation (a false positive caused by structural similarity between proteins). The panel is divided into seven major categories. Inhalant allergens include over 50 grass, tree, and weed pollen components (including the major cross-reactive allergen groups PR-10, profilins, and polcalcins), house dust mite proteins (Der p 1, Der p 2, Der p 23), storage mites, moulds (Alternaria, Cladosporium, Aspergillus), and animal dander proteins from cat, dog, horse, and rodents. Insect venom components cover bee and wasp venom proteins including Api m 1, Api m 10, Ves v 1, and Ves v 5 — critical for distinguishing true venom allergy from cross-reactive sensitisation before venom immunotherapy. Food allergens include major protein families from cow's milk (casein, alpha-lactalbumin, beta-lactoglobulin), egg (ovomucoid, ovalbumin), wheat (omega-5 gliadin — the exercise-induced anaphylaxis marker), soy, peanut (Ara h 1, 2, 3, 6, 8, 9), hazelnut, walnut, tree nuts, shrimp, fish, and tropical fruits. Who benefits most: patients with overlapping pollen and food symptoms (oral allergy syndrome), those with suspected insect venom allergy, children being evaluated for first food introduction risks, adults with chronic urticaria or eczema, and anyone whose skin-prick tests have been ambiguous. The test requires no fasting, no antihistamine washout, and no exposure to live allergens on the day — just a standard venous blood draw at any of Mikrochem's five collection sites. Results are available within 7 working days and are presented as a full numerical report. For patients who need help interpreting results, Mikrochem offers laboratory consultations by arrangement. Contraindications: none specific to the blood draw method. Patients on systemic immunosuppressants may have artificially suppressed IgE levels; in such cases, discuss with your prescribing physician. This test measures sensitisation (IgE antibodies) and not necessarily active clinical allergy — results should always be interpreted in clinical context by a qualified allergologist.