Type
Blood Testing
Biomarkers
51
Duration
30 min
Results
14 days
Comprehensive hormone analysis using dried urine to assess hormone balance and identify potential imbalances.
Adrenal and stress hormone profiling tests — including the DUTCH (Dried Urine Test for Comprehensive Hormones) — provide a detailed picture of how your body produces, metabolizes, and eliminates stress hormones and sex hormones throughout the day. Unlike a single blood draw that captures one moment in time, these tests typically collect multiple samples over 24 hours to map your cortisol curve — the natural rise and fall of cortisol that should peak in the morning and decline through the day. The DUTCH test goes further by measuring hormone metabolites, revealing how your body processes hormones through specific enzymatic pathways. This information is invaluable for functional medicine practitioners investigating chronic fatigue, sleep issues, weight resistance, and hormonal imbalances.
Key Details
- Sample Type
- Dried urine (DUTCH) or saliva
- Collection
- Multiple samples over 24 hours
- Turnaround
- 1–3 weeks
- Fasting Required
- No, but timed collection required
- Common Use
- Stress hormone profiling, HPA axis assessment
Who Is This For?
People with chronic fatigue, burnout, or suspected adrenal dysfunction. Those with sleep issues — particularly difficulty waking or evening energy spikes. Women with hormonal imbalances including PCOS, endometriosis, or difficult perimenopause. Anyone working with a functional medicine practitioner who wants detailed hormone metabolism data.
What's Included
Preparation Required
Follow the specific collection instructions included with your test kit. Most adrenal profiles require multiple samples throughout the day (morning, afternoon, evening, night). DUTCH tests use dried urine on filter paper. Saliva cortisol tests require timed samples. Avoid eating, drinking (other than water), or brushing teeth 30 minutes before saliva collection.
Panel Categories
Biomarkers Tested
51Metabolite of estrone.
Ratio of estrogen metabolites.
Metabolite of estradiol.
Metabolite of estrone.
Ratio of estrogen metabolites.
Metabolite of estradiol.
Metabolite of estrone.
Metabolite of progesterone.
Metabolite of progesterone.
Ratio of estrogen metabolites.
Ratio of estrogen metabolites.
Metabolite of estradiol.
Metabolite of estrone.
Metabolite of estradiol.
Metabolite of estrone.
Metabolite of testosterone.
5α-Dihydrotestosterone, a potent androgen.
Metabolite of progesterone.
Neuroactive steroid metabolite of progesterone.
Androstenedione is a steroid hormone and a precursor to testosterone and estrogen. Abnormal levels can indicate adrenal or reproductive disorders.
A metabolite of testosterone.
A steroid hormone produced by the adrenal glands.
Ratio of cortisol to cortisone.
Creatinine levels in the pooled urine sample.
Creatinine levels in the first morning urine sample.
Creatinine levels in the second morning urine sample.
Creatinine levels in the evening urine sample.
Creatinine levels in the night urine sample.
A hormone produced by the adrenal glands, serving as a precursor to testosterone and estrogen. DHEA peaks in early adulthood and declines steadily with age. It's one of the most studied biomarkers in anti-aging research.
A steroid hormone produced by the adrenal glands.
Metabolite of testosterone.
The most potent and prevalent form of estrogen. In women, estradiol regulates the menstrual cycle, fertility, and bone density. In men, it's produced from testosterone and plays roles in bone health and libido. Levels vary significantly with age and menstrual cycle.
A metabolite of testosterone.
Free cortisol levels in the first morning urine sample.
Free cortisol levels in the evening urine sample.
Free cortisol levels in the night urine sample.
Melatonin levels in the first morning urine sample.
Melatonin levels in the second morning urine sample.
Melatonin levels in the evening urine sample.
Melatonin levels in the night urine sample.
Weakest oestrogen, produced in the placenta during pregnancy; has oestrogenic and anti-oestrogenic effects.
Calculation of Oestriol/(Oestradiol + Oestrone).
Oestrogen produced in ovaries and other tissues; levels change with age and menopause.
Ratio of pregnanediol to estradiol.
Metabolite of progesterone.
Ratio of testosterone to epitestosterone.
The primary male sex hormone, also important in women at lower levels. Testosterone regulates muscle mass, bone density, fat distribution, libido, and mood. Levels decline naturally with age in both sexes.
Metabolite of cortisol.
Metabolite of cortisone.
Total cortisol levels in urine.
Total cortisone levels in urine.
- Category
- Diagnostic
- Sample Type
- Dried urine or saliva (varies by test)
- Duration
- 30 min
- Results
- 14 days