Polynucleotides (PDRN Skin Regeneration)

Polynucleotides (PDRN — PolyDeoxyRiboNucleotides) are DNA-fragment derivatives extracted from salmon DNA that stimulate skin cell regeneration at a molecular level. At Eva Clinic, MUDr. Chovančíková injects PDRN preparations into the mid-dermis, where they interact with cell-surface receptors (primarily A2A adenosine receptors) to activate fibroblast proliferation and collagen synthesis. The treatment is particularly well-suited to clients aged 30+ seeking to address the accelerating collagen breakdown phase of skin ageing, post-sun damage regeneration, and neck or décolletage quality improvements. Unlike HA fillers, polynucleotides work through biological signalling rather than volume addition, producing gradual but sustained improvements over a course of 2–3 sessions.

Polynucleotides represent a category of injectable biorevitalisers derived from purified salmon or trout DNA fragments. The active components — polydeoxynucleotides (PDRN) and polydeoxyribonucleotides (PN) — interact with specific cellular receptors to stimulate tissue repair pathways. The primary mechanism involves binding to A2A adenosine receptors on fibroblasts, triggering intracellular signalling cascades that upregulate collagen type I and III synthesis, suppress inflammatory mediators, and promote angiogenesis (formation of new capillaries to improve local tissue perfusion). At Eva Clinic, MUDr. Chovančíková uses polynucleotide preparations that have been clinically validated in randomised controlled trials for photoaged skin, periorbital rejuvenation, and scalp hair quality. Brands used include Plinest, Newest, and similar products with established safety profiles across the European market. The injection technique involves fine-needle intradermal placement in a serial-puncture or micro-deposit pattern, spacing injection points every 1–2 cm across the treatment area. A topical anaesthetic is applied 30 minutes before to minimise discomfort from the intradermal level of placement (somewhat deeper than superficial papule techniques). Treatment zones include the face (full or zonal), neck, décolletage, and hands. Polynucleotides are frequently positioned as an alternative or complement to PRP: they offer a comparable biological stimulus to fibroblast activity without requiring blood draw and centrifugation, making the treatment simpler to administer and more standardised in terms of active component concentration. Unlike HA skinboosters, polynucleotides produce no immediate hydration-based plumping effect — their action is entirely biochemical, with results developing over 4–8 weeks as new collagen is synthesised. A course of 2–3 sessions spaced 3–4 weeks apart produces the clearest benefit. Clients with early photoageing, fine surface lines, loss of skin elasticity, and post-acne inflammation typically respond well. Post-treatment, small injection papules resolve within 24 hours; mild redness for a few hours is the expected reaction. Bruising is uncommon. Contraindications include fish/seafood allergy (salmon DNA source), active autoimmune skin conditions, pregnancy, and breastfeeding.