Polynucleotide (PDRN) Skin Regeneration

Polynucleotide (PDRN) therapy at Estetika by Medfima uses purified salmon-derived DNA fragments injected into the dermis to promote deep cellular repair and skin regeneration. Unlike hyaluronic acid fillers that add volume, PDRN works by stimulating the skin's own repair machinery — activating fibroblasts, increasing collagen and elastin production, and promoting tissue oxygenation. The aesthetic team performs the treatment using fine needles or a cannula depending on the area, with anesthetic cream applied if needed. Separate protocols exist for the eye area and the full face, both at 4 500 Kč per session. Results are noticeable after the first session, with the full regenerative effect accumulating over a series.

Polynucleotide therapy — marketed under abbreviations such as PDRN (polydeoxyribonucleotide) or PN — has gained significant traction in European aesthetic medicine as a biostimulator rather than a filler. The active ingredient is a highly purified fraction of salmon sperm DNA, which after decades of research has been shown to bind purinergic receptors (specifically A2A receptors) on fibroblast cells. This binding triggers a cascade of regenerative processes: upregulation of VEGF (vascular endothelial growth factor), which improves local blood supply; stimulation of fibroblasts to produce type I and type III collagen and elastin; and an anti-inflammatory effect that calms stressed or reactive skin. At Estetika by Medfima in Pardubice, MUDr. Holohlavská and colleagues perform polynucleotide injections as a standalone regenerative protocol or in combination with biorevitalisation or HA fillers for a more comprehensive result. The clinic's two dedicated protocols — one for the delicate periorbital area (around the eye), one for the face excluding eyes — allow precise dosing calibrated to the tissue depth and sensitivity of each zone. An anesthetic cream is applied to the treatment area 20–30 minutes before the procedure to maximise comfort. Injections are administered via fine needle or, in larger areas, a blunt-tipped cannula to minimise bruising. The recommended starting course is 2–4 sessions spaced 2–4 weeks apart, determined by the physician during the initial consultation based on the client's skin condition and treatment goals. Clients with significant sun damage, post-acne scarring, or pronounced fine lines typically benefit from a full four-session course, while those seeking preventive or maintenance care may see excellent results from two. Annual top-up treatments of 1–2 sessions are standard thereafter. Safety profile is excellent in suitable candidates. Contraindications include pregnancy and breastfeeding, active skin infection or inflammation at the injection site, allergy to fish or seafood, and autoimmune conditions — all reviewed at the mandatory pre-treatment consultation. Post-procedure, mild erythema and occasional small papules at injection points resolve within 24–72 hours. Avoid saunas, intensive exercise, and direct sun exposure for 2–3 days. Makeup can generally be applied the following day. Polynucleotide therapy is particularly effective for clients who want genuinely regenerated skin rather than a filled appearance: those dealing with skin laxity without significant volume loss, periorbital hollowing and crepey eye skin, dull or tired-looking complexion, and post-inflammatory hyperpigmentation. The natural-results ethos aligns well with Estetika by Medfima's clinical philosophy of subtle, physician-led enhancement.